%0 Journal Article %T Continuous flow catalysis with a biomimetic copper(II) complex covalently immobilized on graphite felt %+ Institut des Sciences Chimiques de Rennes (ISCR) %A Marion, Ronan %A Muthusamy, Gopinathan %A Geneste, Florence %< avec comité de lecture %@ 0021-9517 %J Journal of Catalysis %I Elsevier %V 286 %P 266-272 %8 2013 %D 2013 %R 10.1016/j.jcat.2011.11.011 %K Catechol oxidase %K Biomimetic %K Flow %K Heterogeneous catalysis %K Copper complex %K Diazonium salts %K Graphite felt %K N-tripodal ligand %Z Chemical Sciences/CatalysisJournal articles %X The catecholase activity of a copper(II) complex coordinated by a tripodal pyrazole-based ligand was investigated in continuous flow catalysis. The covalent immobilization of the complex on the surface was achieved by a two steps method. First, the porous graphite felt support is functionalized by electrochemical reduction of 4-carboxymethyl-benzenediazonium salts. Second, the complex is covalently immobilized by esterification reaction between the COOH-containing linker and a primary alcohol group present on the C6 chain of the ligand. The two steps of the immobilization process were optimized by using nitro-containing molecules and cyclic voltammetry analyses. The copper complex exhibits higher catecholase activity in continuous flow catalysis than in solution with a 50 times lower amount of catalyst, underlining the advantages of the flow procedure. The presence of H2O2 is detected after catalysis, showing that the four-electron reduction of dioxygen to water does not occur unlike the natural enzyme. %G English %2 https://univ-rennes.hal.science/hal-00842808/document %2 https://univ-rennes.hal.science/hal-00842808/file/JCat2012Geneste_HAL-postprint.pdf %L hal-00842808 %U https://univ-rennes.hal.science/hal-00842808 %~ UNIV-RENNES1 %~ CNRS %~ INSA-RENNES %~ ENSC-RENNES %~ ISCR %~ SCR_MACSE %~ SCR_CTI %~ STATS-UR1 %~ UR1-SPM %~ INC-CNRS %~ UR1-UFR-SPM %~ UR1-HAL %~ UR1-SDLM %~ TEST-UNIV-RENNES %~ TEST-UR-CSS %~ UNIV-RENNES %~ INSA-GROUPE %~ UR1-MMS %~ TEST2-HALCNRS %~ TEST3-HALCNRS