In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Implant Dentistry Année : 2012

In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology.

Fabrice Clipet
  • Fonction : Auteur
Sylvie Tricot
Nora Alno
  • Fonction : Auteur
Murielle Massot
  • Fonction : Auteur
Hélène Solhi
  • Fonction : Auteur
Gilbert de Mello
  • Fonction : Auteur

Résumé

OBJECTIVE: The aim of this work was to determine the relevance of Choukroun's platelet-rich fibrin (PRF) in dental implantology by determining the in vitro effects of soluble factors released by PRF clot. We used 3 different cell lines implicated in dental implantology: osteoblast, keratinocyte, and fibroblast. METHODS: Cellular viability, cell proliferation, and gene expression were analyzed using PRF conditioned medium. Three different cells lines were used: SaOS2 (osteoblast), MRC5 (fibroblast), and KB (epithelial cell). RESULTS: The sulforhodamine B assay showed a significant increase in cell number for the undiluted and 1:3 diluted conditioned medium after 24 and 48 hours. There was no effect for the 1:9 dilution. Cell cycle analysis by flow cytometry confirmed the viability test results. After 48 hours, PRF conditioned medium induced gene expression in osteoblasts. Expression of osteopontin and osteocalcin, late osteogenic markers, was observed using reverse transcriptase-polymerase chain reaction (RT-PCR). CONCLUSIONS: This study establishes a model to evaluate, in vitro, the effects of soluble growth factors released by PRF clot. Our work confirmed PRF is useful in stimulating tissue healing and bone regeneration. This work should recommend Choukroun's PRF in numerous implantology clinical applications.

Dates et versions

hal-00817059 , version 1 (23-04-2013)

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Citer

Fabrice Clipet, Sylvie Tricot, Nora Alno, Murielle Massot, Hélène Solhi, et al.. In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology.. Implant Dentistry, 2012, 21 (1), pp.51-6. ⟨10.1097/ID.0b013e31822b9cb4⟩. ⟨hal-00817059⟩
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