Catching and attaching cells using an array of microholes - Université de Rennes Accéder directement au contenu
Communication Dans Un Congrès Année : 2000

Catching and attaching cells using an array of microholes

Résumé

We succeeded in moving and catching cells using an arrayed microholes structure combined with immobilized antibodies layer. One microhole caught one cell. The success rate of catching cell was almost 100%. Cells are moved toward microholes with liquid flow into these microholes. Around the microholes, patterned gold was deposited and antibodies were chemically attached to it. When the cells met the antibodies placed around the microholes on the patterned gold, they attached to them. The cells were first vacuumed by the liquid flowing through the microholes then fixed by the antibody. This is the key technology to improve the efficiency of DNA insertion into cells in gene therapy. The active part of the microsystem is able to inject gene into cell using microcapillary array injector. The microholes insure in that way two functions: first to attract and isolate cells towards the active part by means of aspiration and second to inject gene into cells. The antibodies insure the immobilization part.
Fichier non déposé

Dates et versions

hal-00795228 , version 1 (27-02-2013)

Identifiants

Citer

A. Tixier, L. Griscom, K. Cozic, H. Nagai, Bruno Le Pioufle, et al.. Catching and attaching cells using an array of microholes. 1st Annual International Ieee-Embs Special Topic Conference on Microtechnologies in Medicine & Biology, Proceedings, 2000, France. pp.36-40, ⟨10.1109/mmb.2000.893736⟩. ⟨hal-00795228⟩
71 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More