Biodiversity of coagulase-negative Staphylococci in French cheeses, dry fermented sausages, processing environments and clinical samples. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue International Journal of Food Microbiology Année : 2010

Biodiversity of coagulase-negative Staphylococci in French cheeses, dry fermented sausages, processing environments and clinical samples.

Emmanuel Coton
  • Fonction : Auteur
Marie-Hélène Desmonts
  • Fonction : Auteur
Sabine Leroy
Monika Coton
  • Fonction : Auteur
Emmanuel Jamet
  • Fonction : Auteur
Souad Christieans
  • Fonction : Auteur
Isabelle Lebert

Résumé

In this study, the biodiversity of Coagulase-Negative Staphylococci (CNS) strains isolated in France from cheese related samples (227 isolates) and dry sausage related samples (204 isolates) was compared to the biodiversity of 297 clinical isolates. Species identification was performed using different molecular methods (specific PCR, "Staph array" hybridization and sodA gene sequencing). Infraspecific biodiversity of strains belonging to the main CNS species found in both food and clinical samples was then assessed by pulse-field gel electrophoresis (PFGE). For food-related samples, the main species encountered corresponded to Staphylococcus equorum (28.5%), S. xylosus (28.3%), S. saprophyticus (12.5%) and S. succinus (7.7%); while, for clinical isolates, the main species encountered corresponded to S. epidermidis (69.4%), S. capitis (9.8%), S. hominis (4.5%), S. warneri (4.5%) and S. haemolyticus (3.8%). The two main species common to both food and clinical samples corresponded to S. epidermidis and S. saprophyticus. Concerning infraspecific biodiversity, PFGE profiles of S. equorum, S. saprophyticus and S. epidermidis showed a large genomic biodiversity. Comparatively, S. xylosus exhibited a lower biodiversity. No correlation could be observed between PFGE patterns and either the geographical origin or the sample type. This study highlighted that no food strains had similar PFGE profiles to clinical ones and that the two main food-related species, S. equorum and S. xylosus, were not found in clinical samples. The identification of CNS species and the characterisation of the genetic diversity of the strains constitute a first step towards CNS safety assessment.

Dates et versions

hal-00750792 , version 1 (12-11-2012)

Identifiants

Citer

Emmanuel Coton, Marie-Hélène Desmonts, Sabine Leroy, Monika Coton, Emmanuel Jamet, et al.. Biodiversity of coagulase-negative Staphylococci in French cheeses, dry fermented sausages, processing environments and clinical samples.. International Journal of Food Microbiology, 2010, 137 (2-3), pp.221-9. ⟨10.1016/j.ijfoodmicro.2009.11.023⟩. ⟨hal-00750792⟩
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