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Article Dans Une Revue Biomedical optics express Année : 2011

Skeletal muscle sarcomeric SHG patterns photo-conversion by femtosecond infrared laser.

Gaëlle Recher
Denis Rouède
Emmanuel Schaub

Résumé

Femtosecond laser at 780 nm excitation wavelength was used to photo-convert the physiological sarcomeric single band (SB) second harmonic generation (SHG) pattern into double band (DB) in Xenopus laevis premetamorphic tail muscles. This photo-conversion was found to be a third order non-linear optical process and was drastically reduced at 940 nm excitation wavelength. This effect was no longer observed in paraformaldehyde fixed muscles and was enhanced by hydrogen peroxide. The action of hydrogen peroxide suggests that reactive oxygen species (ROS) could contribute to this photo-conversion. These results demonstrate that sarcomeric DB SHG pattern is a marker of sarcomere photodamage in xenopus tadpole muscles and highlight the need of being very careful at using two-photon excitation while observing living tissues. Moreover they open new avenues for in situ intravital investigation of oxidative stress effects in muscle dysfunctions and diseases.

Dates et versions

hal-00697462 , version 1 (15-05-2012)

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Citer

Gaëlle Recher, Denis Rouède, Emmanuel Schaub, François Tiaho. Skeletal muscle sarcomeric SHG patterns photo-conversion by femtosecond infrared laser.. Biomedical optics express, 2011, 2 (2), pp.374-84. ⟨10.1364/BOE.2.000374⟩. ⟨hal-00697462⟩
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