Collagen and myosin characterization by orientation field second harmonic microscopy. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Optics Express Année : 2008

Collagen and myosin characterization by orientation field second harmonic microscopy.

Résumé

Collagen and myosin fibrils are endogenous harmonophores that both give rise to Second Harmonic Generation (SHG). By combining four polarization SHG images provided by a scanning microscope, we show that the orientation of the principal axis of the nonlinear susceptibility tensor chi(2) can be determined for each pixel of the image. The ratio rho = chi33/chi15 of the principal components of chi(2) of collagen and myosin was obtained with the same method, and found within the range 1.6-1.8 and 0.5-0.6 respectively. The orientation of the principal axis of chi(2) is shown to be correlated to the orientation of the fibrils themselves. This provides a straightforward method, which we call Orientation Field-Second Harmonic Microscopy (OF-SHM), to reconstruct orientation fields of fibrils at various scales and resolutions in different biological systems (from muscle sarcomere to the whole embryo).
Fichier principal
Vignette du fichier
oe-16-20-16151.pdf (2.28 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-00672471 , version 1 (11-05-2023)

Licence

Paternité

Identifiants

Citer

Christophe Odin, Thomas Guilbert, Alia Alkilani, Olena P. Boryskina, Yann Le Grand, et al.. Collagen and myosin characterization by orientation field second harmonic microscopy.. Optics Express, 2008, 16 (20), pp.16151-16165. ⟨10.1364/OE.16.016151⟩. ⟨hal-00672471⟩
154 Consultations
11 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More