Proteomic analysis of the spermatogonial stem cell compartment in dogfish Scyliorhinus canicula L. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics Année : 2010

Proteomic analysis of the spermatogonial stem cell compartment in dogfish Scyliorhinus canicula L.

Résumé

In the dogfish (Scyliorhinus canicula L.) the testicular germinative zone (GZ), composed of large isolated spermatogonia surrounded by elongating pre-Sertoli cells, is located between the albuginea and the ventrolateral intratesticular vessel. During the spermatogenic wave, cysts radiate in maturational order forming distinct testicular zones. In this study, soluble proteins of the GZ and of the zone containing cysts with spermatocytes were separated by two-dimensional electrophoresis. Gel images were matched and then evaluated for GZ-specific proteins. From the1400 protein spots identified, 680 were found to be apparently specific to this zone. Using MALDI-TOF/TOF mass spectrometry, de novo sequences were obtained for 33 proteins out of the 169 selected for identification by mass spectrometry, but only 16 of these 169 proteins were identified. One of them, proteasome subunit alpha-6, was analyzed further by immunohistochemistry. This study demonstrates the utility of the dogfish as a model for proteome analysis of the spermatogonial stem cell niche, even if it remains restricted by the lack of genomic data available on Elasmobranchs.

Dates et versions

hal-00653339 , version 1 (19-12-2011)

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Géraldine Loppion, Régis Lavigne, Charles Pineau, Pierrïck Auvray, Pascal Sourdaine. Proteomic analysis of the spermatogonial stem cell compartment in dogfish Scyliorhinus canicula L.. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 2010, 5 (2), pp.157-64. ⟨10.1016/j.cbd.2010.03.004⟩. ⟨hal-00653339⟩
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