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Article Dans Une Revue Analytical Chemistry Année : 2008

Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents

Résumé

This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays. Use of a thin poly(p-xylylene) (parylene) film having micrometer-sized apertures (φ = 15−50 μm, t = 20 μm) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores; thus, a large number of BLMs could be arrayed without any skillful technique. We optically confirmed the simultaneous formation of BLMs in a 5 × 5 matrix, and in our durability test, the BLM lasted more than 15 h. Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers.

Dates et versions

hal-00599949 , version 1 (11-06-2011)

Identifiants

Citer

Bruno Le Pioufle, H. Suzuki, K. Tabata, H. Noji, S. Takeuchi. Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents. Analytical Chemistry, 2008, 80 (1), pp.328-332. ⟨10.1021/ac7016635⟩. ⟨hal-00599949⟩
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