Generation of stable Xenopus laevis transgenic lines expressing a transgene controlled by weak promoters. - Université de Rennes Accéder directement au contenu
Article Dans Une Revue Transgenic Research Année : 2009

Generation of stable Xenopus laevis transgenic lines expressing a transgene controlled by weak promoters.

Résumé

Combining two existing protocols of trangenesis, namely the REMI and the I-SceI meganuclease methods, we generated Xenopus leavis expressing a transgene under the control of a promoter that presented a restricted pattern of activity and a low level of expression. This was realized by co-incubating sperm nuclei, the I-SceI enzyme and the transgene prior to transplantation into unfertilized eggs. The addition of the woodchuck hepatitis virus posttranscriptional regulatory element in our constructs further enhanced the expression of the transgene without affecting the tissue-specificity of the promoter activity. Using this combination of methods we produced high rates of fully transgenic animals that stably transmitted the transgene to the next generations with a transmission rate of 50% indicating a single integration event.
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Dates et versions

hal-00416493 , version 1 (14-09-2009)

Identifiants

Citer

Anne L'Hostis-Guidet, Gaëlle Recher, Brigitte Guillet, Abdulrahim Al-Mohammad, Pascal Coumailleau, et al.. Generation of stable Xenopus laevis transgenic lines expressing a transgene controlled by weak promoters.. Transgenic Research, 2009, 18 (5), pp.815-27. ⟨10.1007/s11248-009-9273-0⟩. ⟨hal-00416493⟩
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