Infection with influenza virus induces IL-33 in murine lungs. - UFR SVE Sciences de la vie et de l'environnement Accéder directement au contenu
Article Dans Une Revue American Journal of Respiratory Cell and Molecular Biology Année : 2011

Infection with influenza virus induces IL-33 in murine lungs.

Résumé

IL-33, a novel IL-1 family member, is crucially expressed and involved in pulmonary diseases, but its regulation in viral diseases such as influenza A virus (IAV) remains unclear. This study aimed to characterize the expression and release of IL-33 in lungs of IAV-infected mice in vivo and in murine respiratory epithelial cells (MLE-15) in vitro. Our results provide evidence of up-regulation of IL-33 mRNA in IAV-infected murine lungs, compared with noninfected control mice. The overexpression of IL-33 was positively correlated with a significant increase in mRNA encoding the proinflammatory cytokines TNF-α, IFN-γ, IL-1β, and IL-6, and was also associated with an increase in IFN-β mRNA. A profound overexpression of IL-33 protein was evident in IAV-infected murine lungs and bronchoalveolar lavages of influenza-infected mice, compared with low concentrations in naive lungs in vivo. Immunolocalization highlighted the cellular expression of IL-33 in alveolar epithelial and endothelial cells, along with increased infiltrate cells in virus-infected lungs. Further in vitro experiments showed an induction of IL-33 transcript-in MLE-15 cells and human epithelial cells (A549) infected with different strains of IAV in comparison with noninfected cells. In conclusion, our findings evidenced a profound expression of IL-33 in lungs during both in vivo and in vitro IAV infections, suggesting a role for IL-33 in virus-induced lung infections.
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Dates et versions

hal-00681982 , version 1 (29-05-2020)

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Ronan Le Goffic, Muhammad Imran Arshad, Michel Rauch, Annie L'Helgoualc'H, Bernard Delmas, et al.. Infection with influenza virus induces IL-33 in murine lungs.. American Journal of Respiratory Cell and Molecular Biology, 2011, 45 (6), pp.1125-32. ⟨10.1165/rcmb.2010-0516OC⟩. ⟨hal-00681982⟩
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