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Article Dans Une Revue Journal of Phycology Année : 2008

Two-dimensional gel electrophoresis analysis of brown algal protein extracts

Résumé

High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms.

Dates et versions

hal-00653296 , version 1 (19-12-2011)

Identifiants

Citer

Andrés Ritter, Geraldine Dennett, Freddy Boehmwald, Nathalie Guitton, Charles Pineau, et al.. Two-dimensional gel electrophoresis analysis of brown algal protein extracts. Journal of Phycology, 2008, 44 (5), pp.1315-1321. ⟨10.1111/j.1529-8817.2008.00575.x⟩. ⟨hal-00653296⟩
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