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Article Dans Une Revue Scientific Reports Année : 2017

Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin.

Résumé

Unusual intramolecular cross-links present in adhesins from Gram-positive bacteria have been used to develop a generic process amenable to biotechnology applications. Based on the crystal structure of RrgA, the Streptococcus pneumoniae pilus adhesin, we provide evidence that two engineered protein fragments retain their ability to associate covalently with high specificity, in vivo and in vitro, once isolated from the parent protein. We determined the optimal conditions for the assembly of the complex and we solved its crystal structure at 2 Å. Furthermore, we demonstrate biotechnological applications related to antibody production, nanoassembly and cell-surface labeling based on this process we named Bio Molecular Welding.

Dates et versions

hal-01485073 , version 1 (08-03-2017)

Identifiants

Citer

Julie Bonnet, J. Cartannaz, G. Tourcier, Carlos Contreras-Martel, Jean-Philippe Kleman, et al.. Autocatalytic association of proteins by covalent bond formation: a Bio Molecular Welding toolbox derived from a bacterial adhesin.. Scientific Reports, 2017, 7, pp.43564. ⟨10.1038/srep43564⟩. ⟨hal-01485073⟩
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